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| Registros recuperados: 21 | |
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| Pigné, Sandrine; Zykwinska, Agata; Janod, Etienne; Cuenot, Stéphane; Kerkoud, Mohammed; Raulo, Roxane; Bataillé-simoneau, Nelly; Marchi, Muriel; Kwasiborski, Anthony; N’guyen, Guillaume; Mabilleau, Guillaume; Simoneau, Philippe; Guillemette, Thomas. |
| Background: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. Results: Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Äabmak1 strain... |
| Tipo: Text |
Palavras-chave: Monooxygenase; Cell wall; Melanin; Fungus; Flavoprotein. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00371/48189/48305.pdf |
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| Vriesmann,Lúcia Cristina; Petkowicz,Carmen Lúcia de Oliveira; Carneiro,Paulo Irajara Borba; Costa,Maria Eugênia; Beleski-Carneiro,Eliana. |
| The aim of this work was to study the acidic polysaccharides of Psidium cattleianum. Pectic fractions were obtained by successive extractions with water, EDTA and sodium carbonate (4 and 25ºC). Monosaccharide composition, total carbohydrates, uronic acids and protein contents were determined for each fraction. The yield of water and EDTA-soluble fractions and high content of uronic acid were consistent with the presence of pectins probably arising from the middle lamellae. On the other hand, sodium carbonate-soluble pectins had a higher neutral sugar content, indicating highly branched polysaccharides in these fractions, consistent with pectins from cell wall. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pectins; Acidic polysaccharides; Psidium cattleianum; Cell wall. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200001 |
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| Pessoa,José Dalton Cruz; Calbo,Adonai Gimenes. |
| According to the model frequently used as reference, plant cell growth occurs only when turgor surpasses a threshold. This model was proposed considering a cylindrical cell of constant wall thickness immerged in a water solution, with viscoelastic behaviour, hydraulic conductivity, variable extensibility, and unidirectional elongation. The author, Lockhart (1965), did not consider the effects of apoplasm hydrostatic potential, a subject treated later by Calbo and Pessoa (1994) who argued that this component of cell potential would interfere with cell growth rate. To evaluate this effect, where possible the same deductive procedures as those employed by Lockhart were used here, which resulted in a set of equivalent equations for cell growth, turgor and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoplasm; Cell wall; Growth; Lockhart; Model; Plant. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100003 |
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| Oliveira, Mauro Dal Secco; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Rêgo, Aníbal Coutinho do; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Sforcini, Mariana Paula Rossi; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Freitas Junior, José Esler; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Santos, Juliana dos; Instituto Federal do Rio Grande do Sul; Carvalho, Marina Vieira de. |
| The experiment was conducted to evaluate the bromatological characteristics and the in vitro digestibility of four sugarcane varieties, subjected or not to hydrolysis, with quicklime. A completely randomized design was employed with three replications arranged in a 4 × 2 factorial scheme, with four sugarcane varieties (SP 52454, RB 867515, RB 855536 and IAC 862480), hydrolyzed or not. There was significant effect on brix (p < 0.05) and industrial fiber (p < 0.05), and IAC 862480 variety had the lowest levels of industrial fiber. There were no significant difference (p > 0.05) in neutral detergent fiber, acid detergent fiber and lignin levels among the sugarcane varieties under analysis and for the sugarcanes, hydrolyzed or not. The use... |
| Tipo: Análise laboratorial |
Palavras-chave: 5.04.03.02-8 calcium oxide; Cell wall; Fiber; Ruminants; Saccharum officinarum L.. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/13775 |
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| Labate,Mônica T. Veneziano; Bertolo,Ana L. Ferreira; Nascimento,Daniela Defávari do; Gutmanis,Gunta; Andrade,Alexander de; Rodrigues,Maria J. Calderan; Camargo,Eduardo L.O.; Boaretto,Luis Felipe; Moon,David H.; Bragatto,Juliano; Labate,Carlos A.. |
| UDP-glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in the biosynthesis of plant cell wall polysaccharides. A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. The 1443-bp-ORF encodes a protein of 480 amino acids with a predicted molecular weight of 53 kDa. The recombinant protein expressed in Escherichia coli catalyzed the conversion of UDP-Glc to UDP-GlcA, confirming that the cloned cDNA encodes UGDH. The deduced amino acid sequence of the cDNA showed a high degree of identity with UGDH from several plant species. The Southern blot assay indicated that more than one copy of UGDH is present... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: UDP-glucose; UDP-glucuronate; Hemicellulose; Pectin; Cell wall. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400015 |
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| Redgwell,Robert; Fischer,Monica. |
| This review summarises recent advances in the chemistry, physiology and molecular properties of coffee carbohydrates with a particular focus on the cell wall polysaccharides. The results of detailed chemical studies have demonstrated novel structural features of both the galactomannans and the arabinogalactan polysaccharides of the green and roasted coffee bean. For the first time immunological probes based on monoclonal antibodies for specific polysaccharide epitopes were used to reveal the patterns of distribution of the galactomannans, arabinogalactans and pectic polysaccharides in the coffee bean cell wall. Finally, the results of physiological and molecular studies are presented which emphasise the growing awareness of the potential role the metabolic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; Cell wall; Beverage quality; Seed. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100012 |
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| Ogawa, Y.. |
| Impact of postharvest and/or food processing (such as flour milling to agricultural and food-resource materials) on the digestibility of foodstuffs was investigated through a simulated gastro-intestinal in vitro digestion technique. The differences among the starch digestibilities of cooked rice grain and slurry were examined. In vitro digestion techniques are commonly applied to starch-based milled materials such as flours used for bread making. However, rice is not usually milled to flour but cooked as a whole for consumption. Therefore, maintaining an intact structure of cooked grain during in vitro digestion is very important to evaluate its starch digestibility. Two hundred grams of polished rice grain were cooked using electric rice cooker with 300... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grain; Slurry; Tissue structure; Cell wall; Starch; In vitro digestion; Japan. |
| Ano: 2015 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/3139 |
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| De Paula,A.C.C.F.F.; Sousa,R.V.; Figueiredo-Ribeiro,R.C.L.; Buckeridge,M.S.. |
| ß-Glucans are soluble fibers with physiological functions, such as interference with absorption of sugars and reduction of serum lipid levels. The objective of the present study was to analyze the distribution of ß-glucans in different tissues of the African grass species Rhynchelytrum repens and also to evaluate their hypoglycemic activity. Leaf blades, sheaths, stems, and young leaves of R. repens were submitted to extraction with 4 M KOH. Analysis of the fractions revealed the presence of arabinose, glucose, xylose, and traces of rhamnose and galactose. The presence of ß-glucan in these fractions was confirmed by hydrolyzing the polymers with endo-ß-glucanase from Bacillus subtilis, followed by HPLC analysis of the characteristic oligosaccharides... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SS-Glucans; Cell wall; Hypoglycemic activity; Diabetes mellitus; Grasses; Rhynchelytrum repens. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600010 |
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| Sparo,Mónica; Delpech,Gastón; Batisttelli,Susana; Basualdo,Juan Ángel. |
| The aim of this study was to investigate the immunomodulatory properties of cell wall extract from Enterococcus faecalis CECT7121, measuring the induction of cytokines TNF-α, IL-6, IL-10 and IL-12 from human peripheral blood mononuclear cells (PBMCs). Cell wall extract was prepared from their growth in brain heart infusion broth (18 h, 35 °C). Subsequently, toxicity of the obtained cell wall extract was tested in Balb-C mice. PBMCs were isolated from buffy coats at the Blood Transfusion Service of Hospital Ramón Santamarina (Tandil, Argentina). PBMCs were purified using standard Ficoll-Paque gradient centrifugation. Aliquots of purified leukocytes were incubated at 37 °C for 24 h with heat-killed E. faecalis CECT7121 and cell wall extract. Concentrations... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: E. faecalis CECT7121; Cell wall; Immunomodulationa. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500551 |
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| Lidija,Begović; Lepeduš,Hrvoje; Lalić,Alojzije; Štolfa,Ivna; Jurković,Zorica; Kovačević,Josip; Cesar,Vera. |
| ABSTRACT The aim of this study was to investigate anatomical characteristics of barley (Hordeum vulgare L.) internodes and to reveal the connection with activity of guaiacol-peroxidases, their isoenzyme profiles, lignin, and cellulose content. Three lower internodes were sampled at elongation stage and differed in their anatomical features by showing that lignin deposition follows developmental pattern. Epidermis and hypodermis in the first internode were characterized by continuous layer of the lignified cells while in the second and third internode thin walled parenchyma cells could be observed in the hypodermis. These observations were in accordance with increment of peroxidase activity from the first to the third internode. Two acidic and 2 basic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lignin; Cell wall; Internode; Hordeum vulgare L.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000300352 |
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| Registros recuperados: 21 | |
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